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KMID : 0903519850280040233
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 4 p.233 ~ p.238
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Characteristics and Applications of Immobilized Glucoamylase
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Abstract
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Glucoamylases catalyze a stepwise hydrolysis of starch with the production of glucose. In order to make an efficient conversion of starch into glucose, glucoamylases prepared from Rhizopus spp.(Sigma Co.) were attached to a porous glass and immobilized by glutaraldehyde-induced crosslinking. The porous glass used in this study was ZrO₂ coated. 40∼80 mesh, 550Å pore diameter. Using the forgoing glass, we could couple as much as 50㎎ of protein per gram of carrier. Substrate for the glucoamylase was an enzyme-modified thin-boiling 30% cornstarch solution used where greater solubility and low viscosity are desired. Immobilized glucoamylase had an optimum pH 7.0 to the alkaline side of soluble enzyme. Km values of immobilized and soluble enzyme were 1.04mM and 1.25mM, respectively. The thermal stability of glucoamylase was increased by immobilization and the immobilized enzyme showed am optimum temperature at 40∼60℃. The continuous conversion of cornstarch to glucose by use of immobilized glucoamylase resulted in the production of a more than 90 DE product.
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